Microscopy

The IMM Microscopy Platform is a service available to the entire scientific community, comprising two components: Optical Microscopy and Electron Microscopy. Projects carried out within this platform come from IMM teams, as well as from other regional teams and companies. The biological systems studied are mainly bacteria, viruses, giant viruses, amoebae, micro-algae, as well as macromolecules etc.
Scientific Director: Dr James Sturgis.

Microscopy Team

Artemis KOSTA
Technical Manager

Hugo LE GUENNO
Engineer

Charbel ABOU BOUTROS
M2 Intern

Contact the platform

Microscopy platform areas of expertise

Electron microscopy (TEM, Cryo-EM, SEM)

Super-resolution microscopy (3D-SIM)

Confocal microscopy

Correlative microscopy (CLEM)

Electron microscopy (TEM, Cryo-EM, SEM)

Super-resolution microscopy (3D-SIM)

Confocal microscopy

Correlative microscopy (CLEM)

Microscopy platform services

Sample preparation

- Conventional preparations: chemical fixation, dehydration, resin embedding, ultramicrotomy, section contrast, negative contrast, immunolabeling
- Cryo: cryo-fixation, cryo-substitution, cryo-ultramicrotomy, tokuyasu. vitrification

Imaging department

- Cryo-EM, Cryo-Tomography, TEM, Tomography, CLEM, 3DSIM, TIRF, CLSM, WideField, Image analysis

Consulting and training

- Individual training in various techniques and instruments.
- Theoretical and practical training (masters, doctorates, engineers, researchers): Cryo-EM, Optics, 3DSIM, Fiji...

Cryo ultramicrotome (UC7, FC7 cryo chamber, cryosphere, Leica camera/monitor)
Cryo sample holders (Gatan Elsa, 626)
Epifluorescence (Axio Vert 200M microscope)
Evaporator, glow-discharge (Cressington 208c)
Confocal (Olympus FV1000, FLIM and FCS Pico Quant)
High-pressure freezer (HPF Leica HPM100)
Super-resolution microscope (3D SIM, 2D SIM, 2D SIM-TIRF, TIRF, Lasers 405, 488, 568, and 640 nm)
Vitrification robot (FEI Vitrobot Mark IV)
Scanning electron microscope (Tescan VEGA3 SBU, Low- Vacuum)
Transmission electron microscope (FEI Tecnai G2 20 TWIN (200KV), LaB6, Gatan Oneview 4kx4k)

Reservations

The Microscopy platform in videos

Imaging :

Structural analysis :

Featured publications

2025

Evolutionarily conserved grammar rules viral factories of amoeba-infecting members of the hyperdiverse Nucleocytoviricota phylum. Proc Natl Acad Sci U S A. 2025 Sep 2;122(35):e2515074122.

Rigou S, Schmitt A, Moreno AB, Lartigue A, Danner L, Mayer L, Giry C, Trabelsi F, Belmudes L, Olivero-Deibe N, Le Guenno H, Couté Y, Berois M, Legendre M, Jeudy S, Abergel C, Bisio H.

2025

CP12 controls ribulose 1,5-bisphosphate recycling and carbon acquisition in Chlamydomonas reinhardtii. BioRxiv. 2025.

Gérard C, Lebrun R, Verthuy C, Le Guenno H, Kosta A, Guérard F, Chang KS, Avilan L, Gakière B, Jin ES, Maberly SC, Gontero B, Launay H.

2025

Enderscope.py: A library for computational imaging using the EnderScope automated microscope. SoftwareX 2025; 31: 102210

Gharbi S, Poiraud E, Le Guenno H, Grandgirard E, Rousseau C, Burke N, Mutterer J, Rousseau D.

2025

Comparative analysis of transcriptomic profiles of mesenchymal stem cells at the onset of senescence and after exposure to acute exogenous oxidative stress. Biochem Biophys Res Commun 2025; 754: 151506.

Outskouni Z, Prapa S, Goutas A, Klagkou E, Vatsellas G, Kosta A, Trachana V, Papathanasiou I.

2025

Halophilic archaea produce wax esters and use an alternative fatty acyl-CoA reductase for precursor synthesis. ISME J 2025; Feb 24: wraf035.

Grossi V, Cuny P, Militon C, Witwinowski J, Eddhif B, Sylvi L, Nowakowski M, Kosta A, Antheaume I, Cornil J, Dubrac S, Kende J, Gribaldo S, Borrel G.

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