Microscopy

The IMM Microscopy Platform is a service available to the entire scientific community, comprising two components: Optical Microscopy and Electron Microscopy. Projects carried out within this platform come from IMM teams, as well as from other regional teams and companies. The biological systems studied are mainly bacteria, viruses, giant viruses, amoebae, micro-algae, as well as macromolecules etc.
Scientific Director: Dr James Sturgis.

Microscopy Team

Artemis KOSTA
Technical Manager

Hugo LE GUENNO
Engineer

Artemis KOSTA
Technical Manager

Contact the platform

Microscopy platform areas of expertise

Electron microscopy (TEM, Cryo-EM, SEM)

Super-resolution microscopy (3D-SIM)

Confocal microscopy

Correlative microscopy (CLEM)

Electron microscopy (TEM, Cryo-EM, SEM)

Super-resolution microscopy (3D-SIM)

Confocal microscopy

Correlative microscopy (CLEM)

Microscopy platform services

Sample preparation

- Conventional preparations: chemical fixation, dehydration, resin embedding, ultramicrotomy, section contrast, negative contrast, immunolabeling
- Cryo: cryo-fixation, cryo-substitution, cryo-ultramicrotomy, tokuyasu. vitrification

Imaging department

- Cryo-EM, Cryo-Tomography, TEM, Tomography, CLEM, 3DSIM, TIRF, CLSM, WideField, Image analysis

Consulting and training

- Individual training in various techniques and instruments.
- Theoretical and practical training (masters, doctorates, engineers, researchers): Cryo-EM, Optics, 3DSIM, Fiji...

Cryo ultramicrotome (UC7, FC7 cryo chamber, cryosphere, Leica camera/monitor)
Cryo sample holders (Gatan Elsa, 626)
Epifluorescence (Axio Vert 200M microscope)
Evaporator, glow-discharge (Cressington 208c)
Confocal (Olympus FV1000, FLIM and FCS Pico Quant)
High-pressure freezer (HPF Leica HPM100)
Super-resolution microscope (3D SIM, 2D SIM, 2D SIM-TIRF, TIRF, Lasers 405, 488, 568, and 640 nm)
Vitrification robot (FEI Vitrobot Mark IV)
Scanning electron microscope (Tescan VEGA3 SBU, Low- Vacuum)
Transmission electron microscope (FEI Tecnai G2 20 TWIN (200KV), LaB6, Gatan Oneview 4kx4k)

Reservations

The Microscopy platform in videos

Imaging :

Structural analysis :

Featured publications

2024

Pegylated liposome encapsulating docetaxel using microfluidic mixing technique: Process optimization and results in breast cancer models. Int J Pharm. 2024 May 10 ;656:124091. doi : 10.1016/j.ijpharm.2024.124091. Epub 2024 Apr 6. PMID : 38588758.

Dacos M, Immordino B, Diroff E, Sicard G, Kosta A, Rodallec A, Giacometti S, Ciccolini J, Fanciullino R.

2024

Assembly of a unique membrane complex in type VI secretion systems of Bacteroidota. Nat Commun 15, 429.

Bongiovanni TR, Latario CJ, Le Cras Y, Trus E, Robitaille S, Swartz K, Schmidtke D, Vincent M, Kosta A, Orth J, Stengel F, Pellarin R, Rocha EPC, Ross BD, Durand E.

2023

Structure of a heteropolymeric type 4 pilus from a monoderm bacterium. Nat Commun. 2023 Nov 6 ;14(1):7143. doi: 10.1038/s41467-023-42872-5. PMID: 37932265 ; PMCID: PMC10628169.

Anger R, Pieulle L, Shahin M, Valette O, Le Guenno H, Kosta A, Pelicic V, Fronzes R.

2023

Acinetobacter type VI secretion system comprises a non-canonical membrane complex. PLoS Pathog. 2023 Sep 28 ;19(9):e1011687. doi : 10.1371/journal.ppat.1011687. PMID: 37769028 ; PMCID: PMC10564176.

Kandolo O, Cherrak Y, Filella-Merce I, Le Guenno H, Kosta A, Espinosa L, Santucci P, Verthuy C, Lebrun R, Nilges M, Pellarin R, Durand E.

2023

Collective magnetotaxis of microbial holobionts is optimized by the three-dimensional organization and magnetic properties of ectosymbionts. Proc Natl Acad Sci U S A. 2023 Mar 7 ;120(10):e2216975120. doi: 10.1073/pnas.2216975120. Epub 2023 Feb 27. PMID: 36848579.

Chevrier DM, Juhin A, Menguy N, Bolzoni R, Soto-Rodriguez PED, Kojadinovic-Sirinelli M, Paterson GA, Belkhou R, Williams W, Skouri-Panet F, Kosta A, Le Guenno H, Pereiro E, Faivre D, Benzerara K, Monteil CL, Lefevre CT.

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