Welcome to the Protein Expression and Purification Facility site

Saturday 31 March 2012 by Webmaster

IM2B» Core «Facility

Cloning

Engineering genes,vectors constructs and clones.

Expression & Production

Multi-factorial soluble protein expression screening.

Antigen and polyclonal Antibody Production in collaboration with the on campus animal facility.

Purification

Protein Purification by affinity or exploitation of protein physical/ chemical properties.

Protein A affinity purification of polyclonal antibodies.

Refolding on-line purification of insoluble proteins.

Protein-Protein Interaction

Protein Pull down

DLS

Biolayer interferometry

Optimisation and Quality Control

Stability buffer screening.

Validation of protein correct folding

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