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Protein Production
https://www.youtube.com/watch?v=KtDGkbiXfr0&list=PLhk31IqVPY96UXlRAOGLuz_PyGCFnpGDp&index=6
« Biomoleculer Interaction »
https://www.youtube.com/watch?v=BQ5w16umWEI&list=PLhk31IqVPY96UXlRAOGLuz_PyGCFnpGDp&index=6
Project Propositions
Cloning
Engineering genes,vectors constructs and clones.
Expression & Production
Multi-factorial soluble protein expression screening.
Antigen and polyclonal Antibody Production in collaboration with the on campus animal facility.
Purification
Protein Purification by affinity or exploitation of protein physical/ chemical properties.
Protein A affinity purification of polyclonal antibodies.
Refolding on-line purification of insoluble proteins.
Protein-Protein Interaction
Protein Pull down
DLS
Biolayer interferometry
Optimisation and QualityControl
Stability buffer screening.
Validation of protein correct folding
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