Proteomics

Picro Plateforme balance Protéomique IMM

The IMM proteomics platform offers services in proteomics, quantitative proteomics, mass spectrometry and Edman sequencing in the fields of microbiology, biochemistry and biotechnology. We specialize in quantitative proteomics using state-of-the-art mass spectrometry and liquid chromatography technologies. Our tools and expertise guarantee reliable, reproducible data even for very small quantities of biological material. We offer tailor-made solutions to accelerate your research projects, in compliance with ISO 9001 -NFX 50-900 standards.

As early as 1980, IMM developed a Microsequencing and Amino Acid Analysis Service on the CNRS campus to identify and characterize membrane and soluble proteins in microorganisms. The platform was a pioneer in the field of biological mass spectrometry in Marseille, and one of the first to be awarded the RIO (Réseau Inter Organisme) label. In the early 2000s, thanks to its leadership in proteomics research, IMM also became a founding member of Marseille-Nice-Génopole. In 2008, IMM's proteomics platform became a component of "Marseille Proteomics"(MaP), including 2 other Marseille sites, the CRCM (IPC) and the MCT-PIT2 (La Timone), and was designated an Aix-Marseille University technology platform in 2013.

MaP-IMM's expertise covers fundamental research on the proteins of bacteria, viruses, diatoms, algae, fungi and plants to study various aspects such as enzymatic activities, growth, energy regulation, adaptation to stress and virulence, in relation to environmental and health issues.

Scientific Director: Dr Olivier Genest.

Proteomics Team

Régine LEBRUN
Research engineer
Technical manager

Christophe VERTHUY
Engineer

Maya BELGHAZI
Research engineer

Proteomics Team

Régine LEBRUN
Research engineer
Technical manager

Christophe VERTHUY
Engineer

Maya BELGHAZI
Research engineer

Contact the platform

Proteomics platform areas of expertise

Protein identification by mass spectrometry

Quantitative
proteomics

Protein sequence analysis by Edman sequencing

mass measurement
of intact proteins

Proteomics platform areas of expertise

Protein identification by mass spectrometry

Quantitative proteomics

Protein sequence analysis by Edman sequencing

mass measurement
of intact proteins

Services offered by the
Proteomics platform

The expertise of IMM's proteomics platform is called upon for both R&D projects and scientific services.

- Bacterial Hsp90 chaperone: comprehensive identification of clients to reveal Hsp90 functional roles ANR Bact90ome ANR-20-CE44-0017- 02(2021-2025) Projet leader : Olivier Genest (Bioénergétique et Ingénierie des Protéines, IMM, CNRS UMR7281, Aix-Marseille Université), Partner 2: Régine Lebrun, MaP-IMM CNRS FR3479, Aix-Marseille Université, Partner 3: JeanPhilipe Nougayrède, IRSD, INSERM, UMR 1220, Toulouse

- OCEANIA: iOta Carbonic anhydrasE: A key respoNse to CO2 In diAtoms ANR "OCEANIA" ANR-21- CE20-0029- 03(2022-2025) Project leaders : Véronique Receveur-Brechot & Gontero Brigitte, Bioenergetics and Protein Engineering, BIP2 IMM, CNRS UMR7281, Aix-Marseille Université, Partner 2: Mirjam Czjzek, LBIMM, UMR8227, Roscoff, Partner 3 : Régine Lebrun, MaP-IMM, CNRS FR3479, Aix-Marseille Université

- Target G4P : Identifying the targets and regulation of ppGpp signalling in plants ANR Target G4P22-CE20-0033 (2022-2026) Projet leader : Ben Field, BIAM, CNRS UMR7283, Aix-Marseille Université, Partner 2 : Boyuan Wang, UTSW Texas, Partner 3 : Etienne Maisonneuve, LCB, IMM, Aix-Marseille Université, Partner 4 : Régine Lebrun MaP-IMM, CNRS FR3479, Aix-Marseille Université

Services

- Identification of proteins (soluble or membrane proteins) by LC-MS/MS request form
- Quantitative proteomics analysis request form
- N-terminal sequencing of proteins by Edman method request form
- Mass analysis of intact proteins by MALDI-TOF or ESI-Q-TOF request form
- Characterization of post-translational modifications

Consulting and training

The platform can advise you on setting up the most appropriate protocols to answer a biological question. Contact us to arrange a meeting. The platform is regularly involved in university training programs, and in particular in the IM2B Institute.

Q-TOF (Synapt G1, Waters)
Protein sequencer (PPSQ-31B, Shimadzu)
Q-Orbitrap (Qexactive Plus, Thermofisher)
MALDI-TOF (Microflex II, Bruker)

Featured publications

2024

Posttranslational regulation of photosynthetic activity via the TOR kinase in plants.

D'Alessandro S, Velay F, Lebrun R, Zafirov D, Mehrez M, Romand S, Saadouni R, Forzani C, Citerne S, Montané MH, Robaglia C, Menand B, Meyer C, Field B.

2024

Combining metabolic flux analysis with proteomics to shed light on the metabolic flexibility: the case
of Desulfovibrio vulgaris Hildenborough.

Marbehan X, Roger M, Fournier F, Infossi P, Guedon E, Delecourt L, Lebrun R, Giudici-Orticoni MT, Delaunay S.

2024

The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels.

Ramirez-Franco J, Debreux K, Sangiardi M, Belghazi M, Kim Y, Lee SH, Lévêque C, Seagar M, El Far O.

2022

Reduction in Phosphoribulokinase Amount and Re-Routing Metabolism in Chlamydomonas reinhardtii CP12 Mutants. Int. J. Mol. Sci. 2022, 23, 2710.

Gérard, C, Lebrun, R, Lemesle, E, Avilan, L, Chang, K.S, Jin, E, Carrière, F, Gontero, B, Launay, H.

2022

Characterization of the Achromobacter xylosoxidans type VI secretion system (T6SS) and its implication in cystic fibrosis. Front. Cell. Infect. Microbiol, 16 June 2022 Sec. Molecular Bacterial Pathogenesis

Le Goff, M, Vastel, M, Lebrun, R, Mansuelle, P, Diarra, A, Grandjean, T, Triponney, P, Imbert, G, Gosset, P, Dessein, R, Garnier, F, Durand, E.